Gene Cloning of P43 Surface Protein of Toxoplasma gondii Tachyzoite and Bradyzoite (SAG3)
نویسندگان
چکیده
منابع مشابه
Cloning and sequencing of Toxoplasma gondii major surface antigen (SAG1) gene
Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...
متن کاملExpression and Purification of P43 Toxoplasma gondii Surface Antigen
BACKGROUND Toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. Congenital toxoplasmosis is more important during pregnancy for the first time. In this study we expressed and purified P43 Toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. METHODS The recombinant pGEMEX-1 contained Toxoplasma P43 coding...
متن کاملCloning rhoptry protein 1 (ROP1) gene of Toxoplasma gondii (RH) in expression vector
Toxoplasma gondii contain various immunogenic antigens. The most important Toxoplasma antigens are somatic and excreted/secreted antigens. Rhoptry proteins are known as excreted/secreted antigens. These antigens have been proposed as a vaccine candidate against toxoplasmosis. The main objective of the present work was cloning rhoptry protein1 (ROP1) Gene of Toxoplasma gondii (RH) in a cloning...
متن کاملRecombinant scFv Antibodies against P30 Surface Protein of Toxoplasma Gondii
Background & Aims: Toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. The parasite is able to infect all warm-blooded hosts including humans and farm animals. The infection in humans often occurs after the ingestion of raw or undercooked meat containing tissue cysts. Several methods have been applied to detect this parasite in contaminated foods. Reco...
متن کاملexpression and purification of p43 toxoplasma gondii surface antigen
background : toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all species of mammals including man. congenital toxoplasmosis is more important during pregnancy for the first time. in this study we expressed and purified p43 toxoplasma gondii tachyzoite and bradyzoite specific surface antigen. methods : the recombinant pgemex-1 contained toxoplasma p43 codi...
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ژورنال
عنوان ژورنال: Research Journal of Microbiology
سال: 2007
ISSN: 1816-4935
DOI: 10.3923/jm.2007.170.174